Tie Line

A tie line is a horizontal line drawn across a two-phase region of a binary phase diagram to determine the compositions of coexisting phases at a specified temperature or pressure. Its endpoints intersect the phase boundaries, indicating the composition of each equilibrium phase, while the overall mixture composition lies between them. Combined with the lever rule, the tie line enables calculation of the relative amounts of the phases from the distances between the overall composition and each endpoint. This method is widely used to interpret liquid-liquid, solid-liquid, and solid-solid equilibria in materials chemistry, metallurgy, and chemical process design.

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JoVE Journal - Medicine
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Laparoscopic Extracorporeal Knot-Tying for Uterine Vessel Occlusion during Hysterectomy with Cervical Cerclage in Large Uteri

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2025

Here, we introduce a novel approach-Laparoscopic Extracorporeal Knot-Tying for Uterine Vessel Occlusion during Hysterectomy with Cervical Cerclage in Large Uteri. A laparoscopic extracorporeal knot-pusher is used to perform cervical cerclage, achieving complete occlusion of uterine arteries. Thereafter, the corpus uteri are excised to enhance visibility and facilitate the operative field.

Research

JoVE Journal - Medicine

A Novel Arthroscopic Medial Knot-Tying Suture-Bridge Repair with Rip-Stop Technique for Rotator Cuff Tears

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2026

This protocol presents an optimized rotator cuff repair technique that combines the medial knot-tying suture-bridge repair with a rip-stop configuration, designed to enhance cuff healing and minimize the risk of retear.

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

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Cited by 3 •

2014

We introduce an efficient method to retrograde label retinal ganglion cells (RGCs) in adult zebrafish.

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

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Cited by 17 •

2017

The precise localization of Golgi residents is essential for understanding the cellular functions of the Golgi. However, conventional optical microscopy is unable to resolve the sub-Golgi structure. Here we describe the protocol for a conventional microscopy based super-resolution method to quantitatively determine the sub-Golgi localization of a protein.

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

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Cited by 1 •

2019

We present a protocol to functionalize atomic force microscope (AFM) cantilevers with a single T cell and bead particle for immunological studies. Procedures to probe single-pair T cell-dendritic cell binding by AFM and to monitor the real-time cellular response of macrophages to a single solid particle by AFM with fluorescence imaging are shown.

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