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Biology

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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
 

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Article doi: 10.3791/55996
August 10th, 2017

Summary August 10th, 2017

The precise localization of Golgi residents is essential for understanding the cellular functions of the Golgi. However, conventional optical microscopy is unable to resolve the sub-Golgi structure. Here we describe the protocol for a conventional microscopy based super-resolution method to quantitatively determine the sub-Golgi localization of a protein.

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