Ex Vivo Bone Culture
Ex Vivo Bone Culture - Related Videos
06:39
Culture of myeloid dendritic cells from bone marrow precursors
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Cited by 22 •
2008
This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.
12:08
Tracking Mouse Bone Marrow Monocytes In Vivo
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Cited by 6 •
2015
Monocytes are key regulators of innate immunity and play a critical role in the renewal of the peripheral mononuclear phagocytic system and in case of inflammation. This manuscript describes the procedure of real time imaging of the mouse calvaria bone marrow to study the monocyte mobilisation mechanism.
08:49
Models of Bone Metastasis
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Cited by 115 •
2012
Animal models are frequently utilized to study cancer metastasis to bone. In this protocol we will describe two common methods of tumor inoculation for bone metastasis studies and briefly describe some of the analyses utilized to monitor and quantify these models.
10:51
A System for ex vivo Culturing of Embryonic Pancreas
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Cited by 23 •
2012
Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.
04:00
Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia
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2025
In this video, we demonstrate the isolation and culture of bone marrow stromal cells from the long bones of mice. The isolated adherent BMSCs can be used for further analysis.
FAQs
The explant retains osteoblasts, osteoclasts, progenitor cells, and the surrounding extracellular matrix within the same tissue context. Nutrient media support these living components while researchers observe their coordinated contributions to bone formation and remodeling. This preserves cellular relationships that may be difficult to examine when each cell type is studied separately, helping reveal how local interactions influence skeletal tissue behavior.
The surrounding extracellular matrix provides part of the tissue environment in which bone cells and progenitor cells interact. Maintaining this structure allows investigations to consider cellular organization alongside matrix-associated processes, rather than focusing only on isolated cells. Consequently, researchers can examine developmental events such as mineralization and remodeling in a setting that retains important features of bone tissue architecture.
This approach reduces the complexity of a whole organism while retaining living bone tissue and interactions among its resident components. Researchers can therefore examine cellular responses under controlled conditions and relate them to specific genetic, molecular, or environmental influences. The resulting control supports focused analysis of skeletal growth and signaling before findings are evaluated in more complex in vivo studies.
In developmental biology, cultured bone explants can support studies of skeletal development, tissue patterning, mineralization, and bone remodeling. These processes can be examined through the behavior and organization of osteoblasts, osteoclasts, progenitor cells, and the extracellular matrix. Studying several processes within the same experimental platform helps connect cellular activity with larger changes in developing skeletal tissue.
Researchers begin with a bone explant and place it in nutrient media designed to support its living cellular and extracellular components. They then maintain the tissue under controlled laboratory conditions while examining its organization, growth, or responses to selected influences. The specific readout depends on whether the experiment focuses on mineralization, patterning, remodeling, or another developmental outcome.
The method is useful when investigators need to examine how a defined influence affects living bone tissue without the full complexity of an organism. By observing changes in growth, organization, mineralization, or remodeling under controlled conditions, researchers can characterize responses linked to genetic, molecular, or environmental factors. It can also provide an early platform for evaluating candidate treatments before in vivo studies.