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Chapters
- 00:05Title
- 01:21Imaging System Setup
- 03:24Preparation of the Mouse
- 04:55Immersion System Setup
- 06:22Two-photon Imaging Acquisition Setup
- 09:09Image Acquisition and Monitoring
- 09:56Representative 2-photon Imaging of Parenchymal and Vascular Monocytes
- 11:27Conclusion
Monocytes are key regulators of innate immunity and play a critical role in the renewal of the peripheral mononuclear phagocytic system and in case of inflammation. This manuscript describes the procedure of real time imaging of the mouse calvaria bone marrow to study the monocyte mobilisation mechanism.
Tags
Keywords: Real-time Multiphoton ImagingCell TraffickingCell-to-cell Interactions3D EnvironmentImmune Cell MotilityBlood MonocytesBone MarrowMonocyte TraffickingCsf1r-Gal4VP16/UAS-ECFP (MacBlue) MouseMyeloid-specific PromoterVascular NetworkMedullar MonocytesMigratory BehaviorBone Marrow ParenchymaVasculatureCell-to-cell InteractionsBone Marrow Monocyte SubsetsPathological Conditions
