In Vivo Grafting
In Vivo Grafting - Related Videos
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
0 Views •
Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
11:17
Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring
0 Views •
Cited by 9 •
2015
A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.
09:29
Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
0 Views •
Cited by 31 •
2014
Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
0 Views •
2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
07:37
Mouse Models for Graft Arteriosclerosis
0 Views •
Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
FAQs
The key experimental contrast is between intrinsic developmental properties and local environmental cues. A graft may retain behavior expected from its origin, or it may respond to signals at its new host location. Observing which pattern occurs helps investigators determine whether cell fate, pattern formation, migration, or tissue organization depends primarily on donor-intrinsic information, host-derived influence, or their interaction.
Inductive interactions occur when signals exchanged between donor tissue and the host influence developmental outcomes. These signals can alter the fate of cells, redirect pattern formation, affect migration, or reorganize neighboring tissues. Examining these changes shows how one tissue can influence another and helps reveal the communication processes that coordinate developing structures within a living biological environment.
A defined placement gives the graft a specific local context in which to develop and interact with surrounding tissue. Because different locations can provide different developmental cues, the resulting behavior helps reveal the contribution of the host environment. This design is especially useful for testing whether tissue organization reflects local signals, properties retained from the donor, or both.
Graft behavior provides evidence about the range of outcomes a donor tissue can produce under altered conditions. If the tissue changes in response to its host location, the experiment indicates sensitivity to local developmental signals. If it maintains characteristics associated with its origin, the result supports a contribution from intrinsic developmental potential. Comparison with normal development strengthens this interpretation.
A typical experiment begins by selecting donor tissue and identifying a defined location in a living host. The donor is then placed at that site, where its behavior and interactions with surrounding tissue can be examined during development. Investigators compare the graft with normal developmental patterns to assess changes in cell fate, migration, pattern formation, or tissue organization.
This approach is useful when researchers need to study development within a natural biological environment rather than in isolation. It can address embryonic patterning, regeneration, tissue compatibility, and organ formation. By observing how grafted tissue behaves in relation to its host, investigators can connect developmental outcomes to interactions between tissues and to the cues present at particular locations.
Researchers can evaluate whether a graft changes its fate, migrates, contributes to a new pattern, or becomes integrated into host tissue organization. Comparing these outcomes with normal development helps identify inductive interactions and the relative influence of donor and host. The findings can clarify how local signals and intrinsic properties cooperate to coordinate complex developmental processes.