Short Tandem Repeat Analysis
Short Tandem Repeat Analysis - Related Videos
03:01
Detection of Fish Pathogens Using Variable Number Tandem Repeat (VNTR) Analysis
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2025
Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This video demonstrates the procedure for separating fluorescently labeled variable number tandem repeat (VNTR) amplicons, derived from a fish-pathogenic bacterium, using capillary electrophoresis to generate size-specific electropherogram profiles for bacterial identification.
09:39
DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
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Cited by 9 •
2011
Leprosy, caused by Mycobacterium leprae, is still endemic in many places. In order to learn about the spread and mode of transmission of leprosy, it is important to determine which strain of M. leprae has infected a patient. Variable numbers of tandem repeats (VNTR) typing is one such method.
07:09
Tandem Mass Spectrometry
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2023
In tandem mass spectrometry a biomolecule of interest is isolated from a biological sample, and then fragmented into multiple subunits in order to help elucidate its composition and sequence. This is accomplished by having mass spectrometers in series. The first spectrometer ionizes a sample and filter ions of a specific mass to charge ratio. Filtered ions are then fragmented and passed to a second mass spectrometer where the fragments are analyzed. This video introduces the principles of...
10:33
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Cited by 5 •
2019
The Multi-Locus Variable-number tandem-repeat Analysis (MLVA) assay presented here enables inexpensive, robust and portable high-resolution genotyping of the fish-pathogenic bacterium Yersinia ruckeri. Starting from pure cultures, the assay employs multiplex PCR and capillary electrophoresis to produce ten-loci MLVA profiles for downstream applications.
08:09
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
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Cited by 10 •
2015
This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.
FAQs
Variation among short tandem repeat loci creates differences in allele patterns between individuals or cell populations. These differences provide sensitive genetic markers for distinguishing samples that may otherwise be difficult to separate by appearance or developmental behavior. In research, the resulting profile supports sample authentication and helps associate a population of cells with its genetic source.
PCR first amplifies selected short tandem repeat loci so the targeted sequences can be examined in a sample. Capillary electrophoresis then separates the resulting DNA fragments according to size, producing an allele pattern for comparison. Using these steps together converts variation at repeated loci into a profile that can be evaluated across developmental or cellular samples.
Allele patterns provide a comparative genetic signature rather than relying on cellular appearance alone. When profiles from separate samples differ, the patterns can indicate that the populations have different genetic identities or origins. This comparison is especially useful when developmental experiments contain multiple cell populations and researchers need to connect observed behavior with a particular genetic source.
Chimerism studies use genetic profile differences to assess whether a sample contains cells associated with distinct genetic sources. Comparing allele patterns can help distinguish donor-derived cells from host tissues, allowing researchers to evaluate the distribution or persistence of those populations. In developmental biology, this provides a molecular way to relate tissue composition to experimental cell origin.
A typical workflow selects informative short tandem repeat loci, amplifies those loci by polymerase chain reaction, separates the resulting fragments by size using capillary electrophoresis, and compares allele patterns among samples. The comparison produces the basis for identifying matching or distinct genetic profiles. This sequence also supports reproducible authentication when samples are examined across developmental experiments.
The method is useful when researchers must track cell lineage, verify the identity of experimental material, or determine whether cells found in developing or regenerating tissue are donor-derived or host-derived. By linking allele patterns with sample identity, it helps connect cellular behavior to developmental origin and provides genetic context for interpreting tissue formation or regeneration outcomes.