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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

DOI:
10.3791/52827-v

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08:09 min

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September 15, 2015

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1Department of Biology,Concordia University

Chapters

  • 00:05Title
  • 01:06Perform Cell Lysis in Catridge Filter Unit with Cells Preserved in RNA Stabilization Solution
  • 04:07Protein Precipitation and Peptide Extraction
  • 05:25DNA Precipitation
  • 06:49Results: Taxonomic and Functional Analysis of Arctic Ocean Microbial Communities
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.

Tags

Keywords: Aquatic Microbial MetaproteomicsMetaproteomicsMetagenomicsMetatranscriptomicsMarine Microbial CellsSterivex FilterRNA StabilizationPeptide DigestionTandem Mass SpectrometryDatabase Search

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