Tracer Experiment

A tracer experiment is a measurement method that follows a detectable substance through a system to reveal how materials, fluids, or particles move and interact. In engineering studies, researchers introduce a tracer with known properties, monitor its concentration or signal over time, and analyze its arrival, dispersion, and removal to characterize transport behavior. These experiments can determine flow paths, residence-time distributions, mixing efficiency, leakage, and mass-transfer rates in reactors, pipelines, porous media, and environmental systems. By linking measured tracer responses to system models, engineers can validate designs, diagnose performance problems, and improve the control and efficiency of complex processes.

Tracer Experiment - Related Videos

Research

JoVE EoE - Large Animal Models

Tracer Molecule Delivery in the Cisterna Magna: A Method to Deliver Fluorescent Tracer Molecules via Direct Cannulation of the Cisterna Magna in Pig

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2025

This video describes the protocol for tracer molecule delivery via direct cannulation of the cisterna magna in a pig brain. This method helps visualize the distribution of cerebrospinal fluid in the glymphatic system.

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

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Cited by 12 •

2018

This protocol describes an experimental procedure to quantitatively and comprehensively investigate the metabolism of multiple nutrient sources. This workflow, based on a combination of isotopic tracer experiments and an analytical procedure, allows the fate of consumed nutrients and the metabolic origin of molecules synthetized by microorganisms to be determined.

Research

JoVE Journal - Medicine
Free Sample

Automated Radiochemical Synthesis of [18F]3F4AP: A Novel PET Tracer for Imaging Demyelinating Diseases

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Cited by 6 •

2017

We demonstrate the semi-automated radiochemical synthesis of [18F]3F4AP and quality control procedures.

Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons

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Cited by 20 •

2015

The efficacy of intramuscular uptake and retrograde transport of molecules to corresponding motor neurons depends on the location of the injection sites with respect to the motor end plates (MEPs). Here, we describe how to locate MEPs on skeletal muscles to optimise retrograde transport of tracers into motor neurons.

Intramuscular Injections Along the Motor End Plates to Shuttle Tracers Directly into Rat Motor Neurons

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2025

Source: Mohan, R., et al. Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons. J. Vis. Exp. (2015)This video demonstrates the targeted delivery of a fluorescent neuronal tracer into motor neurons via intramuscular injection at the motor end plates (MEPs). It outlines the steps involved in preparing the rat, injecting the tracer, and assessing labeled motor neurons for neurotherapeutic potential.

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