Three Prime Utr Deletion
Three Prime Utr Deletion - Related Videos
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Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs
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Gene deletion mutants generated through homologous recombination are the gold standard for gene function studies. The OSCAR (One Step Construction of Agrobacterium-Recombination-ready-plasmids) method for rapid generation of deletion constructs is described. Agrobacterium mediated fungal transformation follows. Finally, a PCR based confirmation method of gene deletions in fungal transformants is presented.
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Verbal Priming
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Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Human memory seems to work in two broad ways. Like modern computers, the human mind has explicit, or declarative, memory: ask a question, and a person gives the best answer they can. Input a query, and a computer program returns the contents of the relevant parts of its stored memory. Humans also have a second kind of memory system, one not really typical of computers, one that experimental psychologists call implicit. Implicit...
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MicroRNAs (miRNAs) are important regulators of gene expression and have been shown to play a role in numerous biological processes. To better understand miRNA-UTR interactions, we have created a genome-wide collection of 3 UTR luciferase reporters paired with a novel luciferase gene and assay reagent, the LightSwitch system.
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Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
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11:31
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
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Cited by 23 •
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Experimental validation of enhancer activity is best approached by loss-of-function analysis. Presented here is an efficient protocol that uses CRISPR/Cas9 mediated deletion to study allele-specific regulation of gene transcription in F1 ES cells which contain a hybrid genome (Mus musculus129 x Mus castaneus).
FAQs
The main consequence is loss of cis-regulatory information contained within the removed sequence. This may include recognition sites for microRNAs, RNA-binding proteins, polyadenylation signals, or other regulatory elements. Because these features influence messenger RNA behavior after transcription, their removal can alter transcript stability, localization, processing, or translation without changing the gene’s coding sequence.
MicroRNA and RNA-binding-protein sites provide distinct routes for post-transcriptional regulation. Removing them may change how regulatory molecules interact with the messenger RNA, which can modify transcript stability, intracellular localization, processing, or translation. The resulting expression change depends on which sites are deleted and how strongly those regulatory interactions normally influence the transcript.
Polyadenylation signals are regulatory features that can be removed along with other downstream sequence. Their loss may affect messenger RNA processing and the subsequent behavior of the transcript, rather than altering the encoded amino acid sequence directly. Consequently, an observed expression phenotype can reflect changes in RNA maturation or handling instead of a change in protein structure.
A coding-region mutation can directly modify the sequence of the encoded protein, whereas a 3′ UTR deletion primarily tests post-transcriptional control. The deleted region may influence messenger RNA stability, localization, processing, or translation while leaving the protein-coding sequence intact. This distinction helps researchers determine whether a phenotype arises from altered regulation rather than altered protein composition.
The experiment evaluates whether the removed downstream sequence contributes to gene regulation. Researchers can examine consequences for messenger RNA stability, localization, processing, or translation and relate those changes to gene-expression phenotypes. This makes the approach useful for functional analysis of cis-regulatory elements, especially when the coding sequence alone cannot explain altered expression.
Removing a defined 3′ UTR segment provides a functional test of whether that region contributes to regulation. If deletion changes gene-expression behavior, the result supports a regulatory role for sequence features within the segment, such as binding sites or processing signals. Such evidence can help interpret variants whose effects are regulatory rather than protein-coding.
Developmental and disease-related phenotypes can result from altered gene expression even when the encoded protein sequence remains unchanged. A Three Prime Utr Deletion helps connect downstream regulatory sequence to those expression effects by testing its influence on messenger RNA behavior. The resulting evidence can clarify how post-transcriptional control contributes to genetic phenotypes in these contexts.
Interpretation should consider several possible expression-level outcomes rather than focusing only on protein abundance. Loss of the sequence may affect messenger RNA stability, localization, processing, or translation, and these effects can occur through different deleted elements. Linking the observed phenotype to the affected RNA process helps distinguish the contribution of specific cis-regulatory functions.