Zebrafish Imaging
Zebrafish Imaging - Related Videos
09:17
Labeling and Imaging Cells in the Zebrafish Hindbrain
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Cited by 7 •
2010
Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.
08:00
Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
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Cited by 1 •
2014
Imaging of cerebrovascular development in larval zebrafish is described. Techniques to facilitate 3D imaging and modify cerebrovascular development using chemical treatments are also provided.
10:45
Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
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Cited by 33 •
2012
The rapid development, small size and transparency of zebrafish are tremendous advantages for the study of innate immune control of infection1-4. Here we demonstrate techniques for infecting zebrafish larvae using the fungal pathogen Candida albicans by microinjection, methodology recently used to implicate phagocyte NADPH oxidase activity in control of fungal dimorphism5.
09:39
Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
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Cited by 20 •
2011
Dying cells are extruded from epithelial tissues by concerted contraction of neighboring cells without disrupting barrier function. The optical clarity of developing zebrafish provides an excellent system to visualize extrusion in living epithelia. Here we describe methods to induce and image extrusion in the larval zebrafish epidermis at cellular resolution.
07:06
Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos
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Cited by 6 •
2014
Described here is a protocol for semi-automated imaging of tissue-specific fluorescence in zebrafish embryos.
FAQs
Genetically encoded reporters make selected cellular or biological processes visible through fluorescence, allowing researchers to connect gene activity or altered regulation with observable changes. When paired with imaging in living embryos, these reporters can show when and where a phenotype develops rather than only documenting an endpoint. This supports more precise interpretation of gene function during development and organ formation.
Mutant lines provide inherited genetic alterations that can be compared with appropriate controls, while targeted gene manipulation enables researchers to examine the effects of specific genetic changes. Imaging then links those alterations to visible changes in cells, tissues, development, behavior, or disease-related phenotypes. This combination helps distinguish genetic associations from functional consequences within a vertebrate model.
Time-resolved observation can reveal how a genetic alteration changes a process as it unfolds, including developmental progression, organ formation, or the emergence of disease-related traits. Because imaging can be performed in living animals, researchers can relate early cellular or tissue changes to later outcomes. This helps investigate conserved biological mechanisms rather than relying only on a final phenotype.
A typical workflow begins by selecting embryos or fish carrying a mutant line, genetically encoded reporter, or targeted gene alteration. Researchers then use microscopy, particularly fluorescence microscopy when reporters are involved, to visualize relevant cells, tissues, or processes. Comparing the resulting images and phenotypes across genetic conditions can reveal how the alteration affects development, organ formation, behavior, or disease-related biology.
In a genetic screen, visible imaging outcomes provide a way to identify animals or genetic alterations associated with a biological phenotype. Researchers can examine changes in development, organ formation, cellular behavior, or other observable traits and then relate those outcomes to the underlying genetic condition. This approach makes imaging a practical bridge between large-scale genetic variation and functional biological interpretation.
The approach can be used to investigate how genes regulate embryonic development, organ formation, cellular processes, behavior, and disease-related phenotypes. It also supports functional studies that compare genetic alterations with visible outcomes and can help evaluate potential therapeutic strategies. Its value comes from connecting genotype with biological effects in a genetically tractable vertebrate model.