Ficoll-paque Plus
Ficoll-paque Plus - Related Videos
05:52
Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium
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Cited by 46 •
2020
The protocol describes organotypic explants of mouse neuroretina, cultivated together with its retinal pigment epithelium (RPE), in R16 defined medium, free of serum and antibiotics. This method is relatively simple to perform, less expensive, and time-consuming when compared to in vivo experiments, and can be adapted to numerous experimental applications.
06:46
Metabolic Labeling of the Nascent Transcriptome in Xenopus Early Embryogenesis
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2026
We provide detailed methods to metabolically label and purify nascent transcripts for transcriptome analysis in Xenopus early embryos using 5-ethynyl-uridine (5-EU).
09:45
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
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Cited by 17 •
2017
This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.
11:19
Imaging Subcellular Structures in the Living Zebrafish Embryo
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Cited by 8 •
2016
Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.
09:01
A Quantitative Assessment of the Phagocytosis of Allogeneic and Xenogeneic Erythrocytes by Rat Macrophages In Vitro
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2025
This work describes a standardized in vitro protocol to quantify and compare the phagocytosis rates of xenogeneic (human) and allogeneic (rat) red blood cells by isolated rat macrophages.
FAQs
Separation depends on differences in cellular density during centrifugation. Erythrocytes and granulocytes move downward through the medium and sediment, whereas lymphocytes and monocytes remain at the plasma–Ficoll interface. This physical arrangement concentrates the desired mononuclear population into a recoverable layer, providing an enriched starting material for subsequent immunological analyses.
The medium’s controlled density creates the conditions needed for differential movement of blood-cell populations during centrifugation. If erythrocytes and granulocytes sediment while mononuclear cells remain at the interface, researchers can collect the target layer separately from much of the whole-blood cellular material. This enrichment supports more focused characterization of immune cells in downstream experiments.
The interface contains enriched peripheral blood mononuclear cells, principally lymphocytes and monocytes. In contrast, erythrocytes and granulocytes sediment through the density-gradient medium. Recovering this interface therefore provides a preparation centered on cells that are commonly examined for immune phenotyping, functional responses, cytokine analysis, and pathogen-related changes.
The workflow begins with whole blood layered for density-gradient centrifugation. After centrifugation, the mononuclear-cell layer is identified at the plasma–Ficoll interface and carefully recovered. The collected cells are then washed before being directed into downstream applications. This sequence converts a mixed blood sample into an enriched preparation suitable for immunology and infection studies.
Washing follows recovery of the mononuclear-cell layer and prepares the isolated population for subsequent analysis or culture. In the stated workflow, this step occurs before applications such as flow cytometry, cytokine analysis, cell culture, and pathogen-response studies. Maintaining that sequence helps researchers use the recovered cells as a consistent input for immune assays and functional experiments.
Researchers use the isolated mononuclear cells to characterize host immune responses and evaluate changes associated with infection. The preparation can support flow cytometry, cell culture, cytokine analysis, pathogen-response studies, and other immune assays. It also supplies primary cells for molecular and functional experiments, allowing investigators to connect cellular composition with immune activity and infection-related effects.