Germ-free Mouse Colonization
Germ-free Mouse Colonization - Related Videos
07:54
Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
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Cited by 6 •
2011
A progressive colonization procedure is described to further assess its impact on the host hepatic metabolism. Colonization is monitored non invasively by evaluating the urinary excretion of microbial co-metabolites by NMR-based metabolic profiling while hepatic metabolism is assessed by High Resolution Magic Angle Spinning (HR MAS) NMR profiling of intact biopsy.
03:22
Evaluating Intestinal Bacterial Colonization in Germ-Free C. elegans
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2025
Source: Taylor, M. N., et.al. Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions. J. Vis. Exp. (2022)This video demonstrates the exposure of germ-free, age-matched Caenorhabditis elegans worms to a bacterial strain for intestinal colonization. It explains the selective removal of non-adhered and external bacteria, leaving only viable bacteria adhered to the gut epithelium for quantification.
10:41
Germ Cell Transplantation and Testis Tissue Xenografting in Mice
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Cited by 16 •
2012
Protocols for germ cell transplantation and testis tissue xenografting are described. Germ cell transplantation results in donor-derived spermatogenesis in recipient testes and represents a functional reconstitution assay for identification of spermatogonial stem cells (SSCs). Testis tissue xenografting reproduces testis development and spermatogenesis of various donor species in recipient mice.
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
2013
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
07:33
In vitro Organoid Culture of Primary Mouse Colon Tumors
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Cited by 44 •
2013
A simple method to establish primary murine colon tumor organoid is described. This method utilizes the feature that colon tumor cells survive and grow into organoids in media containing limited growth factors, whereas normal colon epithelial do not.
FAQs
The identity of the introduced microorganisms determines which microbial signals the host receives. Those signals can influence immune maturation, barrier function, inflammation, and susceptibility to pathogens. Using a defined community or a selected strain allows researchers to associate particular microbial exposures with specific host responses, making colonization useful for examining how individual members or combinations of microbes affect immunity.
These groups provide distinct microbial exposure conditions for testing causality. Germ-free mice establish a baseline without detectable microbes, colonized mice receive a defined microbial input, and conventionally housed mice provide a broader comparison condition. Differences among their immune or infection-related outcomes can help identify whether particular microorganisms contribute to a response rather than merely correlate with it.
Microbial signals can shape several linked host processes, including immune maturation, epithelial barrier function, inflammation, and vulnerability to pathogens. Examining these outcomes together helps researchers determine whether a microbial exposure primarily changes immune development, strengthens or alters barrier behavior, promotes inflammatory responses, or affects the host's susceptibility during infection-related experiments.
A typical workflow begins with mice raised without detectable bacteria, fungi, or other microbes. Researchers then administer either a defined microbial community or a selected strain under sterile conditions. After exposure, they monitor whether the microorganisms establish and assess host responses. This sequence connects the intended microbial input with subsequent immunological or infection-related outcomes.
The approach is useful when researchers need to examine how microorganisms influence host defense or disease susceptibility. It supports studies of infection mechanisms, host-microbe interactions, immune maturation, barrier function, and inflammation. Because the microbial exposure can be defined, investigators can evaluate how selected organisms affect responses that would be difficult to attribute in a complex, uncontrolled microbial setting.
Colonization studies can reveal relationships between selected microbes and immune or infection-related outcomes, including changes in maturation, barrier function, inflammation, and pathogen susceptibility. These findings can clarify mechanisms of host-microbe interaction and help evaluate the rationale for microbiome-based interventions. Comparisons across microbial exposure conditions also support efforts to distinguish causal effects from associations.