Gold Nanoparticle Coating
Gold Nanoparticle Coating - Related Videos
13:42
Gold Nanoparticle Synthesis
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Cited by 2 •
2021
A protocol for synthesizing ~12 nm diameter gold nanoparticles (Au nanoparticles) in an organic solvent is presented. The gold nanoparticles are capped with oleylamine ligands to prevent agglomeration. The gold nanoparticles are soluble in organic solvents such as toluene.
09:01
A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating
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2016
This article presents a protocol for the production of protein-based nanoparticles that changes the hydrophobic surface to hydrophilic. The produced nanoparticle is an assembly of gliadin-cyanoacrylate diblock copolymers. Spray coating with the produced nanoparticle changes the surface of target material to a hydrophilic surface.
12:31
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Cited by 17 •
2015
A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.
07:08
In Situ Synthesis of Gold Nanoparticles without Aggregation in the Interlayer Space of Layered Titanate Transparent Films
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Cited by 1 •
2017
Here, we present a protocol for the in situ synthesis of gold nanoparticles (AuNPs) within the interlayer space of layered titanate films without the aggregation of AuNPs. No spectral change was observed even after 4 months. The synthesized material has expected applications in catalysis, photo-catalysis, and the development of cost-effective plasmonic devices.
07:23
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
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Cited by 5 •
2016
A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.
FAQs
Changing the identity and density of surface ligands can tune particle dispersion, biological interactions, targeting, and immune responses. These variables also influence how proteins associate with the particle surface and how readily immune cells take up the particles. Consequently, coating design helps researchers adjust the balance between colloidal stability, microbial recognition, and desired immunological activity.
Gold-binding chemistry provides the attachment basis for placing polymers, antibodies, peptides, or other biomolecules on the nanoparticle surface. This connection allows the selected coating to remain associated with the particle while controlling surface stability and biological interactions. The resulting ligand layer is therefore central to translating a bare particle into a system with defined targeting or immune-related properties.
The coating molecule influences which surface functions are emphasized. Polymers can help regulate dispersion and interactions with surrounding proteins, whereas antibodies and peptides can contribute to recognition of microbial targets. Other biomolecules may provide additional biological functions. Selecting among these components lets investigators tailor uptake, targeting, and immune responses to the needs of a particular experiment.
A practical design begins by selecting a surface ligand suited to the intended function, then attaching it through gold-binding chemistry. Researchers can vary coating composition and density to tune dispersion, targeting, protein adsorption, immune-cell uptake, and recognition of microbial targets. These design choices should match the planned use, such as antigen delivery, detection, imaging, or host-pathogen investigation.
Coated particles can be configured to carry surface-associated biological functions relevant to antigen delivery and immune recognition. By adjusting ligand composition and density, researchers can investigate how particle dispersion, cellular uptake, and immune responses change in relation to the delivered antigen. This makes the platform useful for exploring nanoparticle-based vaccine strategies rather than treating the particle surface as an incidental feature.
In infection research, these particles support pathogen detection, targeted imaging, and investigations of host-pathogen interactions. Surface molecules can help direct recognition toward microbial targets, while the coating also influences interactions with proteins and immune cells. The same design principles contribute to studies of antigen delivery and to the development of nanoparticle-based diagnostic, vaccine, or antimicrobial strategies.