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Chapters
- 00:05Title
- 01:41Bead Preparations
- 04:03The First Round of Selection
- 05:36Monitoring and Amplifying Product between Selection Rounds
- 08:40Subsequent Rounds of Selection
- 09:51The AuNP Assay
- 10:33Generation of Aptamers to Cortisol
- 11:57Conclusion
A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.
