Rapid Screening
Rapid Screening - Related Videos
05:46
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
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Cited by 15 •
2014
Here we describe cellular cytotoxicity and single round infectivity assays that allow for the rapid and accurate screening of compounds to determine their cellular cytotoxicity (CC50) and IC50 values against WT and drug resistant HIV-1.
08:34
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
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Cited by 6 •
2015
A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.
05:55
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
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Cited by 4 •
2023
CRISPR-Cas technologies have revolutionized the field of genome editing. However, finding and isolating the desired germline edit remains a major bottleneck. Therefore, this protocol describes a robust method for quickly screening F0 CRISPR-injected zebrafish sperm for germline edits using standard PCR, restriction digest, and gel electrophoresis techniques.
03:23
Cell Death Screening Using DAPI Staining: A Method for Rapid Screening of IR Induced Clonogenic Cell Death in Cancerous Cell Lines
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2023
This video demonstrates the methodology to screen for major cell death profiles in ionizing radiation (IR) induced cancer cell lines using DAPI staining assay. The DAPI-stained nuclei of target cells undergoing cell death exhibit distinct morphologies and can be easily identified by fluorescence microscopy.
02:33
A Rapid Image-Based Assay for Screening Surface-Induced Bacterial Virulence
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2026
Source: Perinbam, K., and Siryaporn, A. A Rapid Image-based Bacterial Virulence Assay Using Amoeba. J. Vis. Exp. (2018)This video demonstrates a rapid, image-based method to assess bacterial virulence by incubating amoebae with surface-attached or planktonic bacteria, applying a calcein-acetoxymethyl ester (calcein-AM) agarose pad, and imaging fluorescence to detect host cell damage.
FAQs
They convert detection of a selected target into a signal that can be observed directly or read by an instrument. The target may be a microbial antigen, nucleic acid, host antibody, or another immune marker. This signal allows many specimens to be assessed quickly, while the type of target determines whether the result primarily reflects the infectious agent or the host response.
A rapid method is designed to provide actionable information quickly, but this efficiency may come with reduced sensitivity or specificity compared with more definitive testing. Consequently, a screening result helps identify specimens or situations needing attention rather than serving as the sole basis for every conclusion. Confirmatory testing can resolve uncertain findings and support more reliable decisions.
Microbial antigens and nucleic acids provide information about features associated with the pathogen, whereas antibodies and other immune markers describe the host response. This distinction affects how researchers interpret a screening signal in infection studies. Selecting one target type over another can therefore support different questions, including identifying possible infection or characterizing immune activity across samples.
A general workflow begins by processing a large set of specimens with a streamlined assay, followed by observing or instrumentally reading the resulting signal. The findings then guide specimen prioritization and determine where follow-up or confirmatory testing is most important. This organized sequence helps laboratories and research teams handle many samples while directing limited resources toward the most informative next steps.
Its speed is valuable when teams need an early view of infection or immune-response patterns across many samples. In surveillance, results can help reveal population-level trends; during outbreak investigation, they can support rapid assessment and prioritization. The information can also guide timely follow-up and intervention while more definitive testing is pursued where needed.
Aggregated screening results can reveal patterns of infection or immune response across populations, not just findings in isolated samples. These patterns support surveillance, outbreak investigation, and decisions about which specimens require confirmatory analysis. In immunology and infection research, the approach therefore connects rapid sample-level signals with broader assessments of disease or host-response activity.