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Journal / Immunology and Infection / Rapid Screening of HIV Reverse Transcriptase and…
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Immunology and Infection

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Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Article DOI: 10.3791/51400 • 05:46 min • April 9th, 2014
April 9th, 2014 •
1, 1
1HIV Drug Resistance Program, National Cancer Institute

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Here we describe cellular cytotoxicity and single round infectivity assays that allow for the rapid and accurate screening of compounds to determine their cellular cytotoxicity (CC50) and IC50 values against WT and drug resistant HIV-1.

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Rapid Screening HIV Reverse Transcriptase Inhibitors Integrase Inhibitors Anti-HIV Drugs Toxicity Drug Resistance New Compounds Infection Inhibition Drug-resistant HIV-1 Strains Cellular Cytotoxicity Assay Efficacy Assay Target Cell Line 96-well Plate Serial Dilutions ATP-dependent Luminescence Assay Luciferase Expression Infectivity Measurement Screening Assay Multiple Compounds Screening Triplicate Screening Efficacy Measurement Toxicity Measurement
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