Rna Degradation Analysis
Rna Degradation Analysis - Related Videos
07:30
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
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Cited by 19 •
2020
This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.
02:11
Proteins: From Genes to Degradation
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2020
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation. Transcription is the synthesis of RNA molecules by RNA...
09:05
Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
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Cited by 70 •
2016
Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).
12:04
RNA Analysis of Environmental Samples Using RT-PCR
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Reverse transcription-polymerase chain reaction (RT-PCR) involves the same process as conventional PCR — cycling temperature to amplify nucleic acids. However, while conventional PCR only amplifies deoxyribonucleic acids (DNA), RT-PCR enables the amplification of ribonucleic acids (RNA) through the formation of complementary DNA (cDNA). This enables RNA-based organisms...
02:58
Regulated Protein Degradation
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2020
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells. Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
FAQs
The key distinction is the distribution of RNA fragment sizes. Samples with stronger high-molecular-weight signals contain more intact material, while degradation shifts the profile toward shorter fragments. Examining this pattern rather than relying only on a single measurement helps reveal whether RNA quality is suitable for downstream gene-expression analysis and whether samples differ in their degree of breakdown.
Comparing integrity profiles across biological samples can expose quality differences that may arise during sample handling. If otherwise comparable samples show different fragment distributions, the variation may indicate unequal RNA preservation rather than a true biological difference. Recognizing these discrepancies supports more cautious interpretation of gene-expression results and helps improve consistency across experiments.
Breakdown changes the RNA molecules available for measurement, so damaged samples can distort gene-expression results. This concern applies to assays such as RT-qPCR, transcriptomic analysis, and sequencing, where RNA quality influences interpretation. Assessing integrity first helps distinguish biological changes associated with infection from differences caused by sample degradation.
Electrophoretic and microfluidic separation are the approaches identified for examining RNA integrity. Both provide a profile of RNA signals across fragment sizes, allowing intact high-molecular-weight material to be distinguished from shorter degradation products. The resulting profile gives researchers a quality assessment before they interpret downstream molecular measurements.
It should be used as a sample-quality assessment before RT-qPCR, transcriptomic, or sequencing studies. Applying it to RNA from infected cells or tissues can reveal whether handling-related damage or unequal sample quality may affect subsequent measurements. This preliminary check supports better selection and interpretation of samples in host-pathogen experiments.
In these studies, RNA integrity profiles provide context for interpreting host-pathogen response measurements. They help researchers identify sample-quality differences that could otherwise be mistaken for infection-associated expression changes. By detecting problematic RNA before molecular analysis, the approach improves the reproducibility of experiments and supports more reliable comparisons among infected biological samples.