Eye Imaging

Eye imaging is the use of optical, photographic, and ultrasound-based methods to visualize ocular structures and function, supporting the diagnosis and monitoring of eye disease. Depending on the modality, instruments capture reflected light from the retina, measure tissue layers through low-coherence interferometry, or transmit sound waves that return echoes from internal structures. Fundus photography, optical coherence tomography, and ocular ultrasound can reveal changes in the retina, optic nerve, lens, and posterior eye that may not be visible during routine examination. In medicine, these techniques guide clinical decisions, track treatment response, and advance research on conditions such as glaucoma, diabetic retinopathy, and retinal degeneration.

Eye Imaging - Related Videos

Research

JoVE Journal - Medicine

In Vivo Confocal Microscopy in the Diagnosis and Management of Dry Eye: A Focus on Imaging Protocols and Interpretation

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2025

This article demonstrates the operation and effect of in vivo confocal microscopy in the diagnosis and treatment of dry eye and investigates the application and outcomes of in vivo confocal microscopy to provide a basis for future research.

Research

JoVE Journal - Developmental Biology
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Long-term Live Imaging of Drosophila Eye Disc

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Cited by 6 •

2017

This protocol describes a detailed method for the long-term ex vivo culture and live imaging of a Drosophila imaginal disc. It demonstrates photoreceptor differentiation and ommatidial rotation within the 10 h period of live imaging of the eye disc. The protocol is simple and does not require expensive setup.

Live Imaging of the Drosophila Pupal Eye Using Fluorescence Microscopy

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2025

Source: Hellerman, M. B., et al. Live-imaging of the Drosophila Pupal Eye. J. Vis. Exp. (2015)The video demonstrates a live-imaging technique to study eye patterning in a Drosophila melanogaster pupa. Green fluorescent protein (GFP)-tagged markers enable visualization of cellular organization and differentiation during the formation of the developing eye structure.

Live-imaging of the Drosophila Pupal Eye

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Cited by 7 •

2015

This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

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Cited by 6 •

2009

Here we describe a protocol to examine the migration of glial cells into the developing Drosophila eye using live microscopic analysis paired with GFP tagged glial cells.

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