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JoVE Journal
Developmental Biology

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Live-imaging of the Drosophila Pupal Eye

Live-imaging of the Drosophila Pupal Eye

Article DOI: 10.3791/52120
January 12th, 2015


Summary January 12th, 2015

This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.

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