Journal

Developmental Biology

Long-term Live Imaging of Drosophila Eye Disc

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Long-term Live Imaging of Drosophila Eye Disc

Long-term Live Imaging of Drosophila Eye Disc

DOI:

10.3791/55748-v

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06:08 min

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May 06, 2017

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Chia-Kang Tsao², Hui-Yu Ku², Y. Henry Sun²

¹Institute of Genomic Sciences,National Yang-Ming University, ²Institute of Molecular Biology,Academia Sinica

Chapters

00:05Title
01:04Disc Dissection
02:43Mounting
04:04Confocal Microscopy
04:48Results: Sustained Photoreceptor Differentiation during 10 h Live Imaging
05:44Conclusion

Summary

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This protocol describes a detailed method for the long-term ex vivo culture and live imaging of a Drosophila imaginal disc. It demonstrates photoreceptor differentiation and ommatidial rotation within the 10 h period of live imaging of the eye disc. The protocol is simple and does not require expensive setup.

Long-term Live Imaging of Drosophila Eye Disc

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Chapters

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