Ferritin Immuno-positive Cells
Ferritin Immuno-positive Cells - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
12:19
Analysis of Cell Cycle Position in Mammalian Cells
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Cited by 71 •
2012
Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.
08:57
Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
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Cited by 13 •
2017
A protocol for the protein quantification in complex biological fluids using automated immuno-MALDI (iMALDI) technology is presented.
10:50
Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
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Cited by 37 •
2010
The cell permeable crosslinker DSP [dithiobis-(succinimidyl propionate)] stabilizes transient and labile interactions in vivo, which allows their isolation using stringent protein complex purification techniques. Here we present a technique for crosslinking cells grown in culture followed by isolation of protein complexes by immunoprecipitation.
FAQs
Ferritin immunoreactivity serves primarily as an indicator of intracellular iron storage and regulation, rather than a standalone description of total tissue iron. The observed signal identifies cells containing detectable ferritin protein, allowing researchers to map where iron-related regulation occurs. This distinction helps prevent overinterpreting a positive cell as a complete measurement of iron status.
Immunohistochemistry produces a colored signal, whereas immunofluorescence generates a fluorescent signal after antibody-based detection. Both approaches identify ferritin-containing cells, but they present the staining pattern differently within tissue or cultured-cell samples. The selected modality therefore influences how researchers visualize ferritin distribution and relate that pattern to cell morphology or additional markers.
Ferritin staining gains biological meaning when researchers examine the shape and appearance of positive cells together with other cellular markers. This combined interpretation helps place ferritin expression within the organization of a tissue and its broader molecular pattern. As a result, investigators can more carefully assess associations with cellular stress, inflammation, pathology, or disease-related changes.
A typical workflow begins with fixed tissue or cultured cells, followed by application of a primary antibody that binds ferritin. A labeled detection system then converts antibody binding into a colored or fluorescent signal. Researchers identify cells showing that signal and evaluate its distribution alongside morphology and other markers to interpret the finding.
Medical researchers examine these cells when they need to investigate iron distribution, cellular stress, inflammation, or changes associated with disease. The staining pattern can be compared across tissue samples or cellular conditions to identify where ferritin-related alterations occur. Such comparisons support studies of pathology and disease mechanisms without treating ferritin positivity alone as a complete diagnosis.
Patterns of ferritin immunoreactivity can contribute to evaluation of potential diagnostic biomarkers by showing how ferritin-associated changes are distributed among cells and tissues. Researchers interpret these patterns with morphology and other markers to determine their relevance to disease-associated changes. This context can strengthen pathology studies by connecting a detectable cellular signal with broader tissue findings.