Micro-electrode Array
Micro-electrode Array - Related Videos
09:27
How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
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Cited by 86 •
2010
This protocol provides the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.
02:05
Measuring the Electrophysiological Activity of Neuronal Networks Using Micro-Electrode Arrays
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2025
The video demonstrates how to use a microelectrode array (MEA) to record the electrophysiological activity of neurons derived from human induced pluripotent stem cells (hiPSCs). Electrophysiological activity from a multiwell MEA containing a neuron-astrocyte co-culture is recorded to detect action potentials from multiple neurons in the network, confirming the successful differentiation of hiPSCs into neurons.
09:48
Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array
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Cited by 2 •
2015
We have developed an in vitro unfolded hippocampus which preserves CA1-CA3 array of neurons. Combined with the penetrating micro-electrode array, neural activity can be monitored in both the longitudinal and transverse orientations. This method provides advantages over hippocampal slice preparations as the propagation in the entire hippocampus can be recorded simultaneously.
04:33
Differentiating Human Induced Pluripotent Stem Cells into Neurons on Micro-Electrode Arrays
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2025
The video demonstrates a method for generating excitatory cortical neurons from human-induced pluripotent stem cells (hiPSCs). Upon doxycycline induction, the hiPSCs overexpress neurogenin-2, a neural-specific transcription factor. By using specific media and co-culturing with astrocytes, this process facilitates the hiPSCs' differentiation into neurons with synaptic connections.
02:39
Building Three-Dimensional Neuronal Networks Coupled to Micro-Electrode Arrays
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2025
This video demonstrates the development of a 3D neuronal culture coupled to a planar microelectrode array with hippocampal neurons. The isolated neurons form a 2D network on the microelectrode array's active area and adhere to glass microbeads in a multi-well plate with a membrane insert. Upon transferring these neuron-coated microbeads to the electrode, they organize into an interconnected 3D neural network.
FAQs
Each electrode samples local voltage changes from nearby neurons, allowing researchers to compare when activity occurs and where it is distributed across the measured surface or tissue. Simultaneous multichannel acquisition makes network patterns visible rather than limiting analysis to a single recording site. This supports investigation of coordinated neural activity and spatially organized responses.
A micro-electrode array can support both observation and intervention, but the two functions answer different questions. Recording captures naturally occurring local voltage changes, whereas stimulation applies controlled electrical pulses to activate neural circuits. Comparing spontaneous and evoked activity can help researchers examine how a network responds to direct input, although the overview does not specify pulse parameters or stimulation protocols.
Closely positioned electrodes sample neighboring regions of a biological preparation. This arrangement helps reveal differences in activity across space while simultaneous channels preserve timing relationships among sites. The resulting dataset can show whether neural responses are localized or distributed, making array measurements useful for network-level questions involving the timing and spatial distribution of electrical activity.
Micro-electrode arrays are used with cultured cells, brain slices, and living nervous systems. These settings allow investigators to examine neural activity across different levels of organization, from developing or maintained cells to tissue circuits and intact nervous systems. The appropriate preparation depends on whether the study targets development, circuit function, sensory processing, or disease-related change.
Recordings can reveal the timing and spatial distribution of activity across a neural preparation. That information is relevant to studies of synaptic communication, neuronal development, sensory processing, and disease-related changes, because researchers can examine how electrical patterns vary across multiple sites. The same measurement capability also supports electrophysiological screening, where neural responses are evaluated across experimental conditions.
These systems combine multichannel neural recording with the ability to deliver controlled electrical stimulation. That combination is relevant to brain-computer interfaces and neural prostheses because it provides two complementary capabilities: monitoring electrical activity and activating neural circuits. In research, arrays therefore serve not only as tools for observing nervous-system function but also as platforms for developing technologies that interact with it.