Neurodegenerative Disorders
Neurodegenerative Disorders - Related Videos
06:30
Quantitative Analysis of Climbing Defects in a Drosophila Model of Neurodegenerative Disorders
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Cited by 119 •
2015
We present an optimized inexpensive and reliable negative geotaxis assay in Drosophila melanogaster as a model for neurodegenerative disorders. Being more sensitive to mild locomotor defects, this assay will help screen for potential genetic interactions and drug targets.
13:32
High Content Screening in Neurodegenerative Diseases
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Cited by 8 •
2012
We describe a methodology combining automated cell culturing with high-content imaging to visualize and quantify multiple cellular processes and structures, in a high-throughput manner. Such methods can aid in the further functional annotation of genomes as well as identify disease gene networks and potential drug targets.
19:50
Interview: Protein Folding and Studies of Neurodegenerative Diseases
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Cited by 2 •
2008
In this interview, Dr. Lindquist describes relationships between protein folding, prion diseases and neurodegenerative disorders. The problem of the protein folding is at the core of the modern biology. In addition to their traditional biochemical functions, proteins can mediate transfer of biological information and therefore can be considered a genetic material. This recently discovered function of proteins has important implications for studies of human disorders. Dr. Lindquist also...
05:16
Detecting Histone Modifications in Yeast Cells with Neurodegenerative Protein Overexpression
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2025
Source: Bennett, S. A., et al., Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models. J. Vis. Exp. (2019).This video demonstrates the procedure of assessing histone modifications in yeast cells overexpressing neurodegenerative proteins. The steps include cell lysis, centrifugation, electrophoresis, membrane transfer, and antibody-based detection of modified histones.
04:37
Detecting Neurodegenerative Disease-Associated Protein α-Synuclein Using ELISA
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2025
Source: Bétemps, D., et al., Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein. J. Vis. Exp. (2015).This video demonstrates the procedure to detect neurodegenerative disease-associated protein α-synuclein in brain tissue from transgenic mice using ELISA. The steps include tissue homogenization, centrifugation, and ELISA detection of α-synuclein protein to quantify its disease-associated forms.
FAQs
These processes are linked aspects of disease biology. When abnormal material builds up or removal systems fail, researchers can investigate how these changes relate to neuronal dysfunction and loss. Studying both processes helps connect molecular observations with clinical decline across diseases such as Alzheimer’s disease and Parkinson’s disease.
Mitochondrial dysfunction and chronic neuroinflammation are examined as interacting contributors rather than isolated findings. Neuroscience studies can ask whether altered cellular energy processes and persistent inflammatory activity coincide with neuronal injury or worsening function. This framing is useful because these disorders commonly involve multiple mechanisms, making single-pathway explanations incomplete.
Disrupted synaptic communication can be related to declining movement, cognition, behavior, or sensory function, depending on the neural systems affected. Examining this process gives researchers a functional link between cellular changes and observable symptoms. It also helps explain why studies measure both neuronal biology and changes in behavior or performance.
A neuroscience investigation may combine cellular models, animal studies, neuroimaging, genetic analysis, and fluid biomarkers. These approaches examine disease biology at different levels, from cellular processes to organism-level changes and measurable signals. Using several methods can help clarify pathways, identify early changes, and support development of diagnostic tools and treatments.
Neuroimaging, genetic analysis, and fluid biomarkers can help researchers search for changes associated with disease before or alongside obvious functional decline. These tools complement cellular and animal studies by connecting biological mechanisms with measurable indicators. Their value lies in supporting earlier detection and more informed evaluation of potential treatments.
Research on these conditions applies cellular models, animal studies, imaging, genetic analysis, and fluid biomarkers to clarify disease pathways and identify early changes. Considering several disorders helps neuroscience examine recurring mechanisms, including abnormal protein handling, mitochondrial dysfunction, inflammation, and disrupted synaptic communication, while relating them to different patterns of functional decline.