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High Content Screening in Neurodegenerative Diseases
JoVE Journal
Medicine
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JoVE Journal Medicine
High Content Screening in Neurodegenerative Diseases

High Content Screening in Neurodegenerative Diseases

DOI:
10.3791/3452-v

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13:32 min

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January 06, 2012

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, ,
1Department of Clinical Genetics,VU University Medical Center, 2Center for Neurogenomics and Cognitive Research,Neuroscience Campus Amsterdam

Chapters

  • 00:05Title
  • 01:20Automated Adherent Cell Culture Process
  • 03:31shRNA Virus Production and Plating onto Assay Plates
  • 05:44Lentiviral Transduction and Neuronal Differentiation of SH-SY5Y Cells
  • 06:57High Content Image Acquisition and Image Analysis
  • 09:27Representative Results of High Throughput Content Screening
  • 13:09Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a methodology combining automated cell culturing with high-content imaging to visualize and quantify multiple cellular processes and structures, in a high-throughput manner. Such methods can aid in the further functional annotation of genomes as well as identify disease gene networks and potential drug targets.

Tags

High Content ScreeningNeurodegenerative DiseasesFunctional AnnotationGenomesMolecular NetworksDrug Target IdentificationGenetic Risk FactorsPathogenic MechanismsFunctional ValidationGenome Wide Association StudyParkinson’s DiseaseCausative VariantPathogenic MechanismAssociated RegionGenesPhenotypesTraditional Cell Biology TechniquesMolecular NetworksGenetic MutationsDisease PhenotypesNetwork ComplexityDrug Approval

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