Sub-cellular Resolution
Sub-cellular Resolution - Related Videos
10:26
AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
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Cited by 39 •
2014
We describe a method to map mechanical properties of plant tissues using an atomic force microscope (AFM). We focus on how to record mechanical changes that take place in cell walls during plant development at wide-field mesoscale, enabling these changes to be correlated with growth and morphogenesis.
06:50
Super-Resolution Live Cell Imaging of Subcellular Structures
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Cited by 13 •
2021
Presented here is a protocol for super-resolution live-cell imaging in intact tissue. We have standardized the conditions for imaging a highly sensitive adult stem cell population in its native tissue environment. This technique involves balancing temporal and spatial resolution to allow for the direct observation of biological phenomena in live tissue.
11:19
Imaging Subcellular Structures in the Living Zebrafish Embryo
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Cited by 8 •
2016
Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.
08:16
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
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Cited by 28 •
2019
Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment.
01:32
Subcellular Fractionation
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2023
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation. Differential Centrifugation Differential centrifugation is...
FAQs
Targeted labeling marks selected neuronal structures or biological components, while precise spatial signal detection helps assign each signal to its correct location. Combining these strategies reduces ambiguity when synapses, dendrites, axons, or organelles lie close together. Researchers can therefore monitor localized molecular or structural changes and relate them more reliably to neuronal communication and plasticity.
These structures contribute different elements to neuronal function, so measuring them as a single cellular signal can obscure where a change occurs. Separating their signals allows researchers to examine whether molecular or structural alterations are associated with a particular neuronal compartment. That distinction helps connect localized cellular events with broader changes in communication, plasticity, and circuit function.
Measurements at this scale can reveal molecular and structural changes within defined parts of neurons rather than only changes across an entire cell. Researchers can examine how those localized alterations relate to synaptic communication, neuronal plasticity, and circuit activity. This creates a mechanistic link between changes in cellular components and the operation of neural systems.
A typical approach combines high-resolution imaging, targeted labeling, and precise spatial signal detection. Imaging provides detailed spatial information, labeling identifies the structures or activity of interest, and signal detection helps localize nearby components separately. Together, these elements produce measurements suited to examining neuronal compartments and organelles while preserving their relationship to cellular function.
Researchers use this level of measurement when they need to connect events inside individual neurons with larger neural outcomes. It is relevant to studies of neuronal development, neurodegeneration, and neural disease, where changes may begin at molecular or structural sites. Examining those sites can help relate cellular mechanisms to altered communication, circuit function, physiology, or behavior.
Sub-cellular measurements provide an intermediate level of evidence between molecular or structural changes and whole-system effects. By localizing changes to neuronal compartments and monitoring associated activity, researchers can assess how cellular mechanisms influence communication and circuit function. Those findings can then support interpretations of physiological and behavioral outcomes in development, neurodegeneration, and neural disease research.