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Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

DOI:
10.3791/59533-v

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08:16 min

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June 06, 2019

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, , , , , , , , , , , ,
1Department of Neuroscience and Department of Cell Biology,Yale University School of Medicine, 2Developmental Biology Program,Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling,University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas,Universidad de Puerto Rico

Chapters

  • 00:04Title
  • 00:34C. elegans Embryo Sample Mounting Preparation
  • 01:48Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Embryonic Imaging
  • 03:12Isotropic High-Resolution Volumetric Data: Cell Lineage Analysis
  • 06:06Results: Representative Cellular Identification and Single Cell Characterization of Neurite Outgrowth Dynamics
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment.

Tags

Keywords: Isotropic Light-sheet MicroscopyCaenorhabditis ElegansEmbryogenesisCell Lineage AnalysisSubcellular ResolutionDiSPIMLive ImagingNeuron MorphologyM9 BufferMethylcelluloseMicromanagerAutofocus

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