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Super-Resolution Live Cell Imaging of Subcellular Structures
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JoVE Journal Biology
Super-Resolution Live Cell Imaging of Subcellular Structures

Super-Resolution Live Cell Imaging of Subcellular Structures

DOI:
10.3791/61563-v

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06:50 min

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January 13, 2021

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,
1Department of Biology,The Johns Hopkins University

Chapters

  • 00:04Introduction
  • 00:38Glass Bottom Cell Culture Dish Preparation
  • 01:10Testes Dissection and Mounting
  • 02:31Live Cell In Situ Male Drosophila Germline Stem Cell (GSC) Imaging
  • 04:15Results: Representative Microtubule Time-Lapse Imaging
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for super-resolution live-cell imaging in intact tissue. We have standardized the conditions for imaging a highly sensitive adult stem cell population in its native tissue environment. This technique involves balancing temporal and spatial resolution to allow for the direct observation of biological phenomena in live tissue.

Tags

Keywords: Super-resolutionLive Cell ImagingSubcellular StructuresFluorophore ProbesPhysiological ConditionCellular ProcessDialysis MembraneTestes DissectionGermline Stem Cell ImagingSuper Resolution MicroscopeTransmitted LightLaser PowerPhoto Bleaching

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