Procesando inicio de sesión ...

Journal / Biology / /

JoVE Journal
Biology

Esto contenido es Free Access.

JoVE Journal Biology

Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection (Video) | JoVE

Contribution of the Na+/K+ Pump to Rhythmic Bursting, Explored with Modeling and Dynamic Clamp Analyses

Journal (Biology)

Contribution of the Na⁺/K⁺ Pump to Rhythmic Bursting, Explored with Modeling and Dynamic Clamp Analyses

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates

Journal (Biology)

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates

Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection (Video) | JoVE

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

Article DOI: 10.3791/200471-v • 02:33 min • June 2nd, 2023

June 2nd, 2023 •

Emily Jie-Ning Yang¹, Istvan R. Boldogh^1,2, Haojie Ji², Liza Pon^1,2, Theresa C. Swayne^1,2

¹Department of Pathology and Cell Biology, Columbia University Irving Medical Center, ²Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center

Tags

MtHyPer7 Ratiometric Biosensor Mitochondrial Peroxide Living Yeast Cells Mitochondrial Dysfunction Neurodegenerative Disorders Musculoskeletal Disorders Cancer Aging Genetically Encoded Biosensor Minimally Invasive Hydrogen Peroxide (H2O2) Mitochondria-targeted HyPer7 (mtHyPer7) Circularly Permuted Fluorescent Protein OxyR Protein Domain CRISPR-Cas9 System Marker-free System Yeast Genome Integration Spinning-disk Confocal Microscope System Quantitative Analysis Oxidative Stress Spatiotemporal Information

X

Simple Hit Counter