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Analyzing Protein Expression in Salidroside Treated MCF-7 Cells by Western Blotting (Video) | JoVE

Salidroside's Mechanism in Suppressing MCF-7 Cell Migration and Proliferation

Article DOI: 10.3791/200541-v • 02:27 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1, 1, 1, 1, 1, 2
1Pathology Department, Suining Central Hospital, 2General Surgery Day Ward, Department of General Surgery, the Third People's Hospital of Chengdu & the Affiliated Hospital of Southwest Jiaotong University

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Salidroside Pharmacological Action Molecular Mechanism Inhibiting MCF-7 Cell Proliferation Inhibiting MCF-7 Cell Migration Anti-carcinogenic Anti-hypoxic Anti-inflammatory PI3K-AKT-HIF-1α-FoxO1 Pathway CCK-8 Assay Cell Scratch Assay Migration Assay Matrigel Invasion Assay Cell Apoptosis Assay Cell Cycle Assay Reactive Oxygen Species (ROS) Ca2+ Na+-K+-ATPase Activity Ca2+-ATPase Activity Protein Expression Levels Gene Expression Levels Western Blot Analysis QRT-PCR Analysis
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