Encyclopedia of Experiments: Immunology
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Transcript
Begin with a flask containing stably transfected mammalian cells in a serum-free medium supplemented with a non-ionic surfactant to prevent cell aggregation.
These cells carry a modified genome containing integrated heavy-and-light-chain genes for therapeutic antibody production.
Introduce tryptone, a nutrient-rich source of peptides and amino acids essential for cellular metabolism.
The cell utilizes these amino acids and peptides, and multiplies, resulting in an overgrowth that supports a higher antibody yield.
The expression of genes in the cells drives the production of heavy and light chain polypeptides.
In the endoplasmic reticulum, these chains fold and dimerize via inter-chain disulfide bonds, forming therapeutic antibodies.
In the Golgi apparatus, antibodies undergo post-translational modifications. They are then packaged into vesicles and released into the medium.
Incubate the cells for a longer duration to increase antibody production.
As cell viability begins to decline, harvest the supernatant.
Filter the supernatant containing therapeutic antibodies and store it at a lower temperature for later use.
