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Neuroscience

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JoVE Journal Neuroscience
Lateral Diffusion og exocytose af membranproteiner i dyrkede neuroner vurderes ved hjælp af Fluorescens Recovery og Fluorescens-tab fotoblegning
Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons
Journal (Neuroscience)
Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons
Patch-Clamp Recording to Study the Electrical Responses of Motor Neurons to Spinal Cord Stimulation (Video) | JoVE
Journal (Neuroscience)
Patch-Clamp Recording to Study the Electrical Responses of Motor Neurons to Spinal Cord Stimulation (Video) | JoVE
 
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Lateral Diffusion og exocytose af membranproteiner i dyrkede neuroner vurderes ved hjælp af Fluorescens Recovery og Fluorescens-tab fotoblegning

Article DOI: 10.3791/3747-v • 11:59 min • February 29th, 2012
February 29th, 2012 •
1, 1, 1, 1
1MRC Centre for Synaptic Plasticity, University of Bristol

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Denne rapport beskriver anvendelsen af ​​levende celler billeddannelse og photobleach teknikker til bestemmelse af overfladen ekspression transportveje og handel kinetik eksogent udtrykt, pH-følsom GFP-mærkede proteiner til plasmamembranen af ​​neuroner.

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Neuroscience Fluorescence Recovery Efter Fotoblegning FRAP konfokal billedbehandling fluorophor GFP Super-ekliptika pHluorin september fluorescens tab i photobleach Flip neuron protein trafik synapse
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