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siRNA Screening at identificere Ubiquitin og ubiquitinlignende System Regulators Biologisk Pathways i dyrkede pattedyrceller

Article DOI: 10.3791/51572-v • 10:43 min • May 24th, 2014
May 24th, 2014 •
1, 1, 1, 2, 1,2
1MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, 2Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee

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Her beskriver vi en metode til at udføre en målrettet siRNA "ubiquitome" skærmen for at identificere nye ubiquitin og ubiquitin-lignende regulatorer af HIF1A-medierede cellulære respons på hypoxi. Dette kan tilpasses enhver biologisk sti, hvor en robust læse ud af reporter aktivitet er tilgængelige.

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Biokemi siRNA screening ubiquitin UBL ubiquitome hypoxi HIF1A High-throughput pattedyrceller luciferasereporter
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