Tridimensional de alginato-grano Cultura de células humanas del adenoma pituitario
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February 18, 2016
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Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate beads.
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Avila-Rodríguez, D., Paisano-Cerón, K., Valdovinos- Ramírez, I., Solano-Agama, C., Ortiz-Plata, A., Mendoza-Garrido, M. E. Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells. J. Vis. Exp. (108), e53637, doi:10.3791/53637 (2016).
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