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Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster

Article DOI: 10.3791/54045-v • 10:01 min • June 27th, 2016
June 27th, 2016 •
1
1Department of Pharmacology, University of Virginia

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This paper describes a method for the measurement of fuel oxidation in Drosophila melanogaster in which trace amounts of specific radiolabeled metabolic substrates are fed to flies. The exhaled radiolabeled CO2 that is a produced from fuel oxidation is collected and measured.

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Carbon Dioxide Production Radiolabeled Substrates Drosophila Melanogaster Fuel Oxidation Energy Metabolism Fruit Flies Fly Pod Assembly Radiolabeled Molecules Experimental Apparatus Blue Food Dye Fly Food Vial
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