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Journal / Immunology and Infection / 方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成…
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Immunology and Infection

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JoVE Journal Immunology and Infection
方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成
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Journal (Immunology and Infection)
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
Journal (Immunology and Infection)
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方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成

Article DOI: 10.3791/54667-v • 10:58 min • March 29th, 2017
March 29th, 2017 •
*1,2, *1,3, 1, 1,4, 1, 1,4
1Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 2Fish Disease Research Unit, University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center, Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover
* These authors contributed equally

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Click here for the English version.

脂质是已知在细胞功能中起重要作用。在这里，我们描述了一种方法，以确定嗜中性粒细胞的脂质组合物，重点对胆固醇水平，同时使用HPTLC和HPLC纯化，获得更好的理解嗜中性粒细胞的胞外圈闭形成的基本机制的。

Tags

免疫学，第121，嗜中性粒细胞，嗜中性粒细胞细胞外陷阱（母语），胆固醇，甲基β环糊精（MβCD），高效液相色谱法（HPLC），高效薄层色谱法（HPTLC）
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