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Biochemistry

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JoVE Journal Biochemistry
Time-løst elektrosprayioniserings Brint-deuterium Exchange-massespektrometri til at studere proteiners struktur og Dynamics
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Time-løst elektrosprayioniserings Brint-deuterium Exchange-massespektrometri til at studere proteiners struktur og Dynamics

Article DOI: 10.3791/55464-v • 09:18 min • April 17th, 2017
April 17th, 2017 •
1, 1, 1, 1, 1, 1,2,3
1Department of Chemistry, York University, 2The Centre for Research in Mass Spectrometry, York University, 3The Centre for Research on Biomolecular Interactions, York University

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Konformationel fleksibilitet spiller en afgørende rolle i protein funktion. Heri, beskriver vi anvendelsen af ​​tidsopløst elektrospray massespektrometri koblet til hydrogen-deuterium gengæld for probing de hurtige strukturændringer, som driver funktion i ordnede og uordnede proteiner.

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Biokemi tidsopløst elektrospray ionisering massespektrometri hydrogen-deuterium udveksling protein dynamik uløseligt uordnede proteiner mikrofluidik
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