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Metoder til klassificering af cytoplasmatiske foci som spædbørn

Article DOI: 10.3791/55656-v • 09:33 min • May 12th, 2017
May 12th, 2017 •
*1,2, *1,2, 1,2,3, 1,2, 1,2, 1,2,4
1Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, 2Department of Medicine, Harvard Medical School, 3Department of Histology and Embryology, Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.
* These authors contributed equally

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Stress Granuler (SG'er) er ikke-membraniske cytoplasmatiske strukturer, der dannes i celler udsat for en række stress. SG'er indeholder mRNA'er, RNA-bindende proteiner, små ribosomale underenheder, translationsrelaterede faktorer og forskellige cellesignalproteiner. Denne protokol beskriver en workflow, der bruger flere eksperimentelle metoder til at detektere, karakterisere og kvantificere bona fide SG'er.

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Cellular Biology RNA granulater stress granulater mRNA translation repression immunfarvning, stress respons
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