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Journal / Biochemistry / Bestemmelse av høy-affinitets…
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Biochemistry

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JoVE Journal Biochemistry
Bestemmelse av høy-affinitets antistoff-antigen-bindingskinetikken ved hjelp av fire Biosensor plattformer
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Bestemmelse av høy-affinitets antistoff-antigen-bindingskinetikken ved hjelp av fire Biosensor plattformer

Article DOI: 10.3791/55659-v • 15:27 min • April 17th, 2017
April 17th, 2017 •
1, 2, 1, 1
1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery, Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science, Columbia University

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Vi beskriver her protokoller for måling av antistoff-antigen-bindende affiniteten og kinetikk ved hjelp av fire som vanligvis brukes biosensor plattformer.

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Biochemistry utgave 122 optiske biosensorer antistoff-antigen-vekselvirkninger bindingskinetikk medisiner overflate-plasmonresonans biolag Interferometry
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