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Developmental Biology

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JoVE Journal Developmental Biology
Middelskala forberedelse af
High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function
Journal (Developmental Biology)
High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
Journal (Developmental Biology)
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
 
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Middelskala forberedelse af

Article DOI: 10.3791/55804-v • 09:16 min • May 30th, 2017
May 30th, 2017 •
1, 1, 1, 2, 1
1Biology Department, University of Massachusetts Boston, 2Department of Bioengineering, University of California, San Diego

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Formålet med denne protokol er at tilvejebringe en retfærdig og billig tilgang til indsamling af Drosophila embryoner i medium skala (0,5-1 g) og fremstilling af proteinekstrakter, der kan anvendes i nedstrøms proteomiske applikationer, såsom affinitetsrensnings-massespektrometri (AP-MS ).

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Udviklingsbiologi udgave 123 ekstrakt lysat protein, Frugt flyve embryo proteomics medium skala rensning bur
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