Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / A Method to Assess Fc-mediated Effector Functions …
JoVE Journal
Immunology and Infection

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Immunology and Infection
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
Journal (Immunology and Infection)
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Journal (Immunology and Infection)
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
 

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Article DOI: 10.3791/56256-v • 04:47 min • February 23rd, 2018
February 23rd, 2018 •
1,2, 1, 1,2, 3
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Infectious Disease, J. Craig Venter Institute

Capítulos

Resumen
Automatic Translation

We describe a method to measure the activation of Fc-mediated effector functions by antibodies that target the influenza virus hemagglutinin. This assay can also be adapted to assess the ability of monoclonal antibodies or polyclonal sera targeting other viral surface glycoproteins to induce Fc-mediated immunity.

Tags

Fc-mediated Effector Functions Influenza Hemagglutinin Specific Antibodies Assess Activation Antibody-dependent Immunity Immunology Vaccinology Technique 24 Hours Material Preparation Data Analysis Mark Bailey Graduate Student Peter Palese Laboratory Transfection DNA Coding Viral Hemagglutinin Transfection Reagent Optimum-reduced Serum Medium Infection A549 Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter