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Journal / Bioengineering / Förbättrad 3D Hydrogel kulturer av primära…
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Bioengineering

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JoVE Journal Bioengineering
Förbättrad 3D Hydrogel kulturer av primära gliaceller för In Vitro modellering av Neuroinflammation
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Förbättrad 3D Hydrogel kulturer av primära gliaceller för In Vitro modellering av Neuroinflammation

Article DOI: 10.3791/56615-v • 09:19 min • December 8th, 2017
December 8th, 2017 •
1,2, 1,2, 1,3, 1,2,4, 3, 1,2,5
1Department of Psychiatry, University of Alberta, 2Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART), University of Alberta, 3Department of Chemical and Materials Engineering, University of Alberta, 4Division of Physical Medicine and Rehabilitation, University of Alberta, 5Centre for Neuroscience, University of Alberta

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Häri, presenterar vi ett protokoll för 3D kulturen i rat brain-derived gliaceller, inklusive astrocyter, mikroglia och oligodendrocyter. Vi visar primär cellkultur, methacrylated (HAMA) hydrogel hyaluronsyresyntesen, HAMAphoto-polymerisation och cell inkapsling och prov bearbetning för confocal och scanning electron mikroskopbilder.

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Bioteknik fråga 130 3D cellkultur hydrogel primära glia astrocyt mikroglia oligodendrocyte neuroinflammation hyaluronsyra HAMA
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