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Journal / Biochemistry / 蛋白质的表征(按尺寸-排除色谱与多角度光散射相结合(SEC-MALS)…
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Biochemistry

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蛋白质的表征(按尺寸-排除色谱与多角度光散射相结合(SEC-MALS)

Article DOI: 10.3791/59615-v • 10:00 min • June 20th, 2019
June 20th, 2019 •
1, 2, 3, 2
1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

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该协议描述了尺寸排除色谱与多角度光散射(SEC-MALS)的结合,用于对溶液中的蛋白质和复合物进行绝对表征。SEC-MALS 确定纯蛋白质、原生寡聚物、异质复合物和修饰性蛋白质(如糖蛋白)的分子量和大小。

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生物化学,第148期,尺寸排除色谱,多角度光散射(MALS),蛋白质表征,分子量,牛血清白蛋白,寡聚物,聚合体,蛋白质-蛋白质复合物,质量控制
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