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Biochemistry

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JoVE Journal Biochemistry
Perfilamento Complexome de alta resolução por Cryoslicing BN-MS Analysis
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Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
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Perfilamento Complexome de alta resolução por Cryoslicing BN-MS Analysis

Article DOI: 10.3791/60096-v • 09:33 min • October 15th, 2019
October 15th, 2019 •
1, 1, 2, 1, 1,3, 1,3,4
1Institute of Physiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany

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Um protocolo versátil de cryoslicing BN-MS usando um micrótomo é apresentado para o perfilamento complexome de alta resolução.

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Bioquímica edição 152 perfilamento complexome complexo proteico interações proteína-proteína eletroforese de gel nativa proteômica funcional proteínas de membrana
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