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Bioengineering

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JoVE Journal Bioengineering
Vækst af menneskelige og får Hornhinde endotelcellelag på biomaterialemembraner
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Vækst af menneskelige og får Hornhinde endotelcellelag på biomaterialemembraner

Article DOI: 10.3791/60762-v • 05:20 min • February 6th, 2020
February 6th, 2020 •
1, 2, 1, 1,3,4,5,6,7,8, 1,3,4
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Science and Engineering Faculty, Queensland University of Technology, 6Faculty of Medicine, University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 8Faculty of Science, University of Western Australia

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Denne protokol beskriver de kritiske skridt, der kræves for at etablere og dyrke hornhinde endotelcellekulturer fra explants af humant eller fårevæv. Der præsenteres også en metode til subkulering af hornhindeeniceller på membranøse biomaterialer.

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Bioengineering hornhinde endotel hornhinde Descemet membran explant biomateriale membran kulturkammer
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