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Immunology and Infection

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Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Article DOI: 10.3791/61462-v • 11:17 min • March 10th, 2021
March 10th, 2021 •
*1,2, *3,4, *2,5, 1,2, 1,2, 2, 2, 4, 1, 4, 1,2
1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences, University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 5Savaid Medical School, University of Chinese Academy of Sciences
* These authors contributed equally

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Resumen
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The protocol describes experimental methods to obtain stable major histocompatibility complex (MHC) class I through potential β2-microglobulin (β2m) substitutions from different species. The structural comparison of MHC I stabilized by homologous and heterologous β2m were investigated.

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Bat MHC Class I Heterologous Beta-2 Microglobulin Hybrid MHC Class I Peptide Presentation Structure And Function Infectious Disease Tumor Immunotherapy E.coli Culture Transformation Plasmid Suspension Heat Shock Lysogeny Broth Antibiotic Resistant Culture Blade Bacterial Clone
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