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Immunology and Infection

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Visualisering av Pseudomonas aeruginosa i sputum hos pasienter med cystisk fibrose

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020
July 16th, 2020 •
1, 2, 1, 1, 1,3, 1,4
1Translational Medicine, Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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Denne protokollen gir metoder for visualisering av bakterieceller og polysakkaridsynteselokus (Psl) polysakkarid i sputum hos pasienter med cystisk fibrose.

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Immunologi og infeksjon utgave 161 kronisk infeksjon lungefunksjon sykelighet antibiotikabehandling pediatriske pasienter romlig organisering aggregering biofilmdannelse polysakkaridproduksjon sputumprøver vevsryddingsteknikk hydrogelmatrise 3D-strukturer fluorescerende etiketter fluorescens in situ-hybridisering anti-PSL-antistoffer eksopolysakkarid DAPI-farging vertsceller høyoppløselig bildebehandling
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