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Journal / Developmental Biology / 从绵羊的成人和前卵巢收集的体外成熟卵母细胞的振动…
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从绵羊的成人和前卵巢收集的体外成熟卵母细胞的振动
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从绵羊的成人和前卵巢收集的体外成熟卵母细胞的振动

Article DOI: 10.3791/62272-v • 06:53 min • July 10th, 2021
July 10th, 2021 •
1, 1, 1, 1, 1
1Department of Veterinary Medicine, University of Sassari

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该议定书旨在为成年和幼羊卵母细胞的玻璃化提供标准方法。它包括从体外成熟介质的准备到暖化后文化的所有步骤。卵母细胞在 MII 阶段使用 Cryotop 进行振动，以确保最低基本体积。

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发育生物学，第173期，冷冻保存，游戏，暖化后文化，生存能力，发展能力，低温梢，线粒体，ROS
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