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Capítulos
- 00:05Introduction
- 00:42Making a Standard Inoculation Box with a Removable Top Lid Mounted with a Mesh
- 01:17Making an Inoculation Chamber and Inoculating Plants in Flats
- 03:13Inoculate Plants with Smaller Inoculation Boxes
- 03:42Inoculate Detached Leaves in Petri Dishes
- 04:37Results: Assessment of Inoculation Evenness
- 05:19Conclusion
We present a protocol for constructing a simple spore-distribution system consisting of an inoculation box with a ~50 µm mesh and a transparent plastic chamber. This can be used to evenly inoculate plants with powdery mildew spores, thereby enabling accurate and reproducible assessment of disease phenotypes of plants under study.
