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Journal / Biochemistry / Ganglioside Extraction, Purification and Profiling
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Biochemistry

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Ganglioside Extraction, Purification and Profiling

Article DOI: 10.3791/62385-v • 10:05 min • March 12th, 2021
March 12th, 2021 •
*1, *1, 1,2
1Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 2Department of Neuroscience, Johns Hopkins University School of Medicine
* These authors contributed equally

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Resumen
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Gangliosides are sialic acid-bearing glycosphingolipids that are particularly abundant in the brain. Their amphipathic nature requires organic/aqueous extraction and purification techniques to ensure optimal recovery and accurate analyses. This article provides overviews of analytic and preparative scale ganglioside extraction, purification, and thin layer chromatography analysis.

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Ganglioside Extraction Ganglioside Purification Ganglioside Profiling Cell Signaling Cell-cell Recognition Ganglioside Isolation Qualitative Analysis Semiquantitative Analysis Lipids Polar Lipids Chloroform Tetrahydrofuran Glass Bottles Teflon-lined Caps Glass Pipettes Stainless-steel Microsyringes Teflon Glass Homogenizers Tissue Wet Weight Methanol Centrifuge
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