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Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism

Article DOI: 10.3791/62982-v • 09:31 min • February 14th, 2022
February 14th, 2022 •
1,2, 3, 1,2, 4, 4, 4, 4, 5, 5, 1,2,6,7, 1,2,6,7
1Division of Cardiac Surgery, Department of Surgery, Faculty of Medicine, University of Alberta, 2Mazankowski Alberta Heart Institute, 3Faculty of Medicine and Dentistry, University of Alberta, 4Department of Surgery, Faculty of Medicine, University of Alberta, 5Ray Rajotte Surgical-Medical Research Institute (SMRI), University of Alberta, 6Alberta Transplant Institute, 7Canadian Donation and Transplantation Research Program

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This paper describes a porcine model of negative pressure ventilation ex situ lung perfusion, including procurement, attachment, and management on the custom-made platform. Focus is made on anesthetic and surgical techniques, as well as troubleshooting.

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Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion Lung Function Metabolism ESLP Device Negative Pressure Ventilation Technology ESLP Research Lung Injury Physiologic Mechanism Safe Preservation Time Donor Lungs Marginal Lungs Transplant Standards Juvenile Pig Lungs Expiratory Tidal Volume Alveolar Recruitment Midline Sternotomy Electrocautery Sternal Saw Sternal Retractor
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